Enzymes in RNA Science and Biotechnology, Volume 691 in the Methods in Enzymology series, highlights new advances in the field, including chapters on Reverse transcriptase Part I (discovery, preparation, general utilization, MarathonRT for routine RT-PCR and for cDNA synthesis on challenging RNA templates Structured RNAs, repeat RNAs and more, Engineering TNA Polymerases Through Iterative Cycles of Directed Evolution, Reverse transcriptase Part II (RNA structure mapping and determination), RNA G-quadruplex (rG4) structure detection using RTS and SHALiPE assays, tRNA Structure-seq in vivo and in droplets, Capture the in vivo intact RNA structurome by CAP-STRUCTURE-seq, and more.

Dr. Chun Kit Kwok completed his PhD in Pennsylvania State University (2014), mentored by Professor Philip C. Bevilacqua and Professor Sarah M. Assmann. Dr. Kwok’s current research focus is to explore the role of RNA structures and interactions in biology, especially the functions of G-quadruplex structures/interactions and non-coding RNA structures/interactions in the mammalian transcriptome and their relevance to gene regulation, RNA metabolism and diseases.
Prof. Ryota Yamagami received his Ph.D degree under the supervision of Prof. Hori at Ehime University in Japan in 2015 where he studied folate-dependent tRNA methyltransferase and tRNA modification network in Thermus thermophilus, a thermophilic bacterium. He joined the Bevilacqua Lab at Pennsylvania State University as a postdoctal fellow in 2016 where he has studied RNA folding.

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